Coding
Part:BBa_K3166000:Design
Designed by: Yijia Ren Group: iGEM19_SEFLS_Shanghai (2019-09-11)
Squalene Synthase(Yarrowia lipolytica)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 135
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 716
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 135
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 135
Illegal NgoMIV site found at 547 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR
Source
It is from Yarrowia lipolytica (ATCC 20362)(kept in the lab).